As well as Rachel's story, the video features two other real life stories from families who have been affected in some way by organ donation, a transplant surgeon, an intensive care consultant and a donor transplant co-ordinator who works with hospital staff and donor families.
Ms Sturgeon said:
"No-one wants to think about their own death but it is important that young people throughout Scotland learn about the realities of organ donation.
"From the age of 12, they can decide for themselves whether they want to donate their organs in the event of their death. By raising awareness of donation and transplantation, dispelling the myths behind the medical science and discussing the ethics, we can ensure they are able to make an informed choice."
Rachel's family wanted to be involved with the education pack to help more young people understand the issue.
Rachel was just seven when her grandfather received a life-saving kidney transplant. She made her parents promise to donate her organs if anything happened to her so, when she passed away, her parents Craig and Sandra agreed to donate her organs. Her kidneys, pancreas and liver were transplanted, saving three lives.
Mrs Warden said:
"Rachel understood organ donation from when she was seven and made me promise we would donate her organs if anything ever happened to her and we fulfilled her wishes.
"It is important for people to discuss organ donation and understand how it can affect people's lives for the better. Educating more young people about the facts of organ donation and raising points for discussion around the ethics and moral issues encourages people to think about what they would like to happen to their organs when they pass away.
"I think it is important you understand organ donation and transplantation and can make an educated decision about your body."
To coincide with the pack's launch, donor transplant co-ordinators will visit more than 60 schools across the country over the next two weeks to present the pack to them and to take part in question and answer sessions with pupils.